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KMID : 1094720080130010084
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Biotechnology and Bioprocess Engineering
2008 Volume.13 No. 1 p.84 ~ p.88
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An In Vitro strategy for the rapid expression of recombinant proteins at low temperatures
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Byun Ju-Young
Kim Dong-Myung
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Abstract
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In this study, we propose a strategy for recombinant protein expression under conditions of low-temperatures. The low-temperature production of recombinant proteins was conducted in a cell-free protein synthesis reaction through the fusion of the N-terminus of the protein of interest with that from a temperature-insensitive protein. For instance, while the expression of hlL-2 or TNF-α was negligible when the reaction temperature was below 20°C, fusion constructs of the N-terminus of these proteins with the N-terminus of CAT allowed for a substantial expression of the fused protein. This method is predicted to provide a useful approach for the expression of unstable and/or aggregation-prone protein.
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KEYWORD
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cell-free protein synthesis, low temperature, PCR
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